Alternative protocol for negative staining using beta vulgaris extract

Natural dyes and pigments are of great importance due to their non-toxic and ecofriendly properties. The colour of Beta vulgaris roots is an important quality characteristic and thus they are regarded as valuable raw material as natural food colourant, additive and for dye production. This study focuses on the use of beetroot extract at various concentrations as a substitute to negative stains such as nigrosine, which are prepared in harmful chemical solvents. The beetroot extract is a cheaper and greener alternative for use in the laboratory over nigrosine