Arginase-1, fibronectin, il-13, tissue inhibitor of metalloproteinase-1 as indicator hepatocellular carcinoma on sprague-dawleyrats induced by diethylnitrosamine and n-morpholine

Background: Hepatocellular carcinoma (HCC) characterized by stromal fibrosis develops in 90% of cases of liver cirrhosis, during the clinical course of chronic liver inflammation. Arginase-1 (Arg-1), fibronectin (FN), IL-13, and the inhibitor of metalloproteinase-1 (TIMP-1) are important regulators of proliferation, differentiation, tumorigenesis.. This study was conducted to investigate the progression of liver fibrosis (cirrhosis) to HCC by inducing diethyl-nitrosamine (DEN) and N-nitrosomorpholine (N-Mor) in Spraque-Dawleyrats. Methods: This study was the experimental design with randomized groups. A total of fourtySprague- Dawley(SD) rats, male, were divided into a control group of ten SD rats, and the treatment group was induced resulting in fibrosis of the liver, and HCC. Statistical analysis was performed by multiregression analysis and Mann Whitney test. Results: This study showed Arg-1, FN, IL-13 and TIMP-1 have strongly and significantly correlate with stromal fibrosis by histopathology (r = -0.870, r = -0.791, r = 0.932, r = 0.808, p = 0.000 respectively). We found that there are significantly difference level of Arg-1 and FN (p=0.041; p 0.002, respectively) between cirrhosis and hepatocellular carcinoma, meanwhile markers of IL-13, TIMP-1 was not significant. Conclusions: The serology marker of Arg-1 or FN could be used as the indicator of HCC.