Assessment of MDA in adjuvant with CA-125 in early ovarian cancer detection

Ovarian cancer (Oca) alone accounts for 16% of all cancers in the female and is the fifth most common form of cancer in the world. As Oca offers poor prognosis, CA-125 is a sole biomarker for the detection of Oca besides histological techniques. CA-125, also known as MUC16 is a membrane bound glycoprotein which is found in higher concentration in blood of Oca patients. In addition to multiple factor endorsing ovarian carcinoma, oxidative stress has also been inscribed to be critical factor in tumor development. The level of oxidative stress on the cells increases as polyunsaturated fatty acid (PUFA) is degraded to generate Malondialdehyde (MDA). The survival percentage of the cell diminishes as the integrity of the plasma membrane slowly falls, leading to apoptosis. MDA can be adopted as supportive biomarker for ovarian cancer along with CA-125 and to confirm, present investigation has been undertaken. LPO (Lipid Peroxidation) assay was performed to measure the MDA level in blood serum collected from 125 ovarian cancer patients with their consent. CA-125 was estimated by ELISA kit method, RBC count and Hemoglobin level were measured by routine method. Mean±S.D of MDA level of OCa patients was higher than normal women, whereas patients of postmenopausal condition had even higher value than premenopausal patients with P<.001. Mean±S.D of CA-125 in patients of postmenopausal condition is lower than premenopausal patients, however both quite higher than normal women with significance <0.0001. RBC count and hemoglobin levels of OCa patients were lower than normal women with p-value <0.0018 and 0.001 respectively. Oxidative stress is directly related with oxygen carrier components and histological study unravels several substantial aspects of anatomy of types of ovarian cancer tissue. Analysis of MDA could directly reflect the total stress on the body and the advancement of the cancer. In the nutshell, analysis of MDA in addition to CA-125 could produce better prognosis and help decide the survival rate and relevant therapy.