Comparative analysis of alkaline protease production by solid state and submerged fermentation

  • strict warning: Non-static method view::load() should not be called statically in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/views.module on line 906.
  • strict warning: Declaration of views_handler_argument::init() should be compatible with views_handler::init(&$view, $options) in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/handlers/views_handler_argument.inc on line 744.
  • strict warning: Declaration of views_handler_filter::options_validate() should be compatible with views_handler::options_validate($form, &$form_state) in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/handlers/views_handler_filter.inc on line 607.
  • strict warning: Declaration of views_handler_filter::options_submit() should be compatible with views_handler::options_submit($form, &$form_state) in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/handlers/views_handler_filter.inc on line 607.
  • strict warning: Declaration of views_handler_filter_boolean_operator::value_validate() should be compatible with views_handler_filter::value_validate($form, &$form_state) in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/handlers/views_handler_filter_boolean_operator.inc on line 159.
  • strict warning: Non-static method view::load() should not be called statically in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/views.module on line 906.
  • strict warning: Non-static method view::load() should not be called statically in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/views.module on line 906.
Author: 
Dr. Nagesh Malik and Vaibhav Shinde
Abstract: 

Enzymes produced from microorganisms that can survive under extreme pH could be particularly useful for commercial applications under high alkaline conditions. In this research project comparative analysis of solid state fermentation and submerged fermentation is carried out, in order to find out which is better of the two. Fungal isolates were screened for alkaline protease production on Skim milk containing agar plates and identified by clear zones of protein hydrolysis around colonies. Totally four fungal isolates were used in primary screening, out of these, one which gave maximum zone, was selected for comparative study of alkaline protease production by solid state and submerged fermentation. Standard media was used for the alkaline protease production and it was observed that solid state fermentation showed almost double the activity than submerged fermentation. This enzyme was found to have optimum activity at pH 10, at pH 11 and pH 12 the enzyme activity was found to decline marginally. Solid state being the cost effective fermentation process with the high activity and high pH range, can be used on the commercial level.

Download PDF: 

CHIEF EDITOR

  

           Prof. Dr. Bilal BİLGİN

Call for Papers - 2017

    submit your paper now

   Vol. 07, Issue 03, March 2017

CURRENT ISSUE

 

Article Tracking

IMPACT FACTOR 2016

          4.753

Get your Certificate

Copyright © 2016 International Journal Development Research. All Rights Reserved.