Cytotoxic effect of chloroform and hexane extracts of arnebia densiflora on neuroblastomacell line
International Journal of Development Research
Cytotoxic effect of chloroform and hexane extracts of arnebia densiflora on neuroblastomacell line
Received 13th September, 2018; Received in revised form 26th October, 2018; Accepted 04th November, 2018; Published online 26th December, 2018
Copyright © 2018, Ayça TAŞ et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objective: We investigated to prepare chloroform and hexane extracts from root parts of Arnebia densiflora (Boraginaceae) plant, to determine cytotoxicity activity on neuroblastoma SH-SY5Y cell lines. Materials and Methods: Arnebia densiflora used in this study were collected from Kangal/Sivas, Turkey at a height of 1540 meters at the stage of full flowering and was identified by Prof. Dr. Akpulat HA, Cumhuriyet University Sivas, Turkey. Dried and powdered roots (5g) were extracted 50mL of chloroform and hexane separately, by maceration method for 24 h in a shaker at room temperature. The prepared extracts were applied to SH-SY5Yneuroblastoma cell line and the cytotoxic effect of these extracts were determined by using MTT method. The spectrophotometric readings at 570 nmwere recorded andanalysed with Graphpad Prism7. Results: In this study, the effects of chloroform and hexane extracts from roots of Arnebia densiflora on the SH-SY5Y cells were compared with the control group and IC50 values were determined for 24, 48 and 72 hours. Conclusion: In this study, it was shown that the effect of chloroform and hexane extracts on SH-SY5Y cells was inhibitory to growth in cancer cells when compared with control group.
