Detection of NS1 antigen from suspected dengue viral infection cases by capture ELISA and immunochromatography methods: a comparative study

×

Error message

User warning: The following theme is missing from the file system: journalijdr. For information about how to fix this, see the documentation page. in _drupal_trigger_error_with_delayed_logging() (line 1138 of /home2/journalijdr/public_html/includes/bootstrap.inc).

International Journal of Development Research

Detection of NS1 antigen from suspected dengue viral infection cases by capture ELISA and immunochromatography methods: a comparative study

Abstract: 

Dengue virus infection is one of the serious mosquito borne viral infection mainly affecting the population from tropical and subtropical countries of the world with high morbidity and mortality. In India it is a major public health problem with all four serotypes of Dengue virus (DENV-1, DENV-2, DENV-3, DENV-4). An estimated 50 million dengue infections occur annually. Among the hospitalized 2.5% of the children die with disease. There is no immunoprophylaxis or specific antiviral therapy available so far. Therefore early, timely and reliable diagnosis is important in patient management, implementation of control measures and for epidemiological purposes.
Aims and objectives: Aim of this study is to detect NS1 antigen, anti-dengue Ig M antibody by ELISA and Immunochromatography (ICT) methods from a single serum sample of clinically suspected cases of dengue virus infection and also to determine the reliability of ELISA and ICT methods to detect the NS1 antigen.
Materials and methods: Total 100 serum samples were collected from clinically suspected and were subjected to NS1, IgM ELISA and NS1, IgM ICT.
Results: Among the 100 serum samples collected, 42 were positive by NS1 ELISA, 70were positive by IgM ELISA and 74 were positive by combination of NS1 and IgM ELISA. The sensitivity and specificity of NS1 ELISA was 42% and 100%, whereas NS1 ICT was 46% and 100% respectively. The sensitivity, specificity of IgM ELISA were 94.5% and100% and IgM ICT were 51.4% and 100%. Conclusion: Combination of antigen and antibody assays on a single sample from a clinically suspected cases is preferable for making a reliable diagnosis. A positive result by ICT suggests dengue infection but a negative result does not rule out the infection which is a major obstacle considering this test as the test of choice for the diagnosis of clinically suspected case of dengue.

 

Download PDF: