Effect of plant growth regulators on in vitro shoot multiplication from callus of Manihot Esculenta Crantz CV. TMS 92/0326

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Author: 
Elian Hubert Dieu Béni, Fotso, Djeuani Astride Carole, Yandia Prosper Simplice, Mbouobda Hermann Desiré and Omokolo Ndoumou Denis
Abstract: 

The aim of this study was to test the effect of plants regulators on shoots multiplication in cassava cv TMS 92/0326 grown in liquid media with addition of 6-bezylaminopurine (BAP), and α-naphthaleneacetic acid (NAA). Nodal segments (1.5 cm height) were used as explants and kept in vitro MS media containing also mio-inositol (100 mg l-1) and sucrose (30 g l-1). The pH of the media was adjusted to 5.8 before autoclaving. The nodal cuttings produced callus. These callus were yellowish and friable. The highest percentage of callogenesis 69.8% was obtained with 0.07 mg l-1 NAA. The highest fresh weights of 1521.2 mg were obtained with 0.07 mg l-1 of NAA. When cultured in the presence of 0.01 to 0.1 mg l-1 BAP, the callus induced shoots. The highest percentages of 82.3% were obtained with 0.07 mg l-1. Also the highest numbers of shoots per callus 27.2 were obtained with 0.07 mg l-1.

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