Objective: The objective of the present study is to evaluate the oral toxicity of the methanolic stem extract of Plumeria acuminata Ait. (a plant used by the Mising tribes of Assam, India to cure reproductive related disorders) and study its effect on the reproductive cycle of female albino mice.
Materials and Methods: The extracts were tested for their effect on the estrous cycle at two dose levels of 200 and 400mg/kg/day. The vaginal smears were then examined twice daily for two weeks and identification of the different phases of the estrous cycle was done. Acute oral toxicity study was done by administering different doses of the extract and observed individually for weight loss, mortality and any other physiological disorder. Results were then compared between the treated to that of the control group.
Result: Results have shown that none of the treated group of animals showed any symptoms associated with toxicity such as dieresis, convulsion, etc. There were also no cases of mortality observed till 72 h after the last dose. The estrous cycle was prolonged in the treated groups with decrease in the number of cycles. There was a marked increase in the diestrous phase while the mestrous phase was significantly decreased.
Conclusion: The methanolic stem extract of P. acuminata Ait. is none toxic and can be considered as safe to experimental animals. The disruption in the normal estrous cycle in mice caused by the extract also is an indication that the plant possesses potent anti-fertility effect.
Prof. Dr. Bilal BİLGİN