Effects of stress induced chronic depression and antidepressant drugs on dentate gyrus of hippocampus in adult albino rats

×

Error message

User warning: The following theme is missing from the file system: journalijdr. For information about how to fix this, see the documentation page. in _drupal_trigger_error_with_delayed_logging() (line 1138 of /home2/journalijdr/public_html/includes/bootstrap.inc).

International Journal of Development Research

Effects of stress induced chronic depression and antidepressant drugs on dentate gyrus of hippocampus in adult albino rats

Abstract: 

The pilot study is conducted using 30 adult albino rats (150-200 gm). They were divided into one control and two experimental groups of equal numbers. Group 1 is Control (Ctrl), Group 2 is Chronic Depression (Ch.Dp) and Group 3 is Treatment Group (Ch.Tt) following Chronic Depression. Ctrl Group received food and water ad-libitum, Ch.Dp Group received Chronic Depression by immobilization method in a Rat Immobilizer for 7 weeks, whereas Ch.Tt Group received anti-depressant drug (Flouxetine 1mg/kg body weight orally) for 4 weeks following depression. All the rats were sacrificed after the experiment. They were perfused using formaldehyde, brains were dissected out and tissue blocks were made by paraffin embedding. Hippocampus was located in the groups (following H and E Staining) and identical areas were compared for neuronal density. Neuronal density was calculated in Dentate Gyrus using Motic images plus 2.0 Software. Neuronal density of control was 110.5 cells/cubic mm which was markedly reduced to 85.4 cells/cubic mm after chronic depression. Neuronal density was enhanced to 144.3 cells /cubic mm in Ch.Tt Group. Statistical analysis was done using students t-test and the significance was assessed. It was found that stress induced depression causes significant neuronal loss in Dentate Gyrus that can be significantly reversed by the pharmacological intervention.

Download PDF: