Evaluation of some plant extracts on mycelial growth and sporulation density of fungal pathogens of groundnut (arachis hypogaea l.) in-vitro
International Journal of Development Research
Evaluation of some plant extracts on mycelial growth and sporulation density of fungal pathogens of groundnut (arachis hypogaea l.) in-vitro
Received 29th April, 2017; Received in revised form 24th May, 2017; Accepted 06th June, 2017; Published online 22nd July, 2017
Copyright ©2017, Patrick M. Ngegba et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The effect of autoclave and ultraviolet light-sterilized aqueous extracts of Tithonia diversifolia, Chromolaena odorata and Tridax procumbens on mycelial growth and sporulation density of fungal pathogens were also determined in vitro. Aqueous extracts of the test plants significantly (p < 0.05) reduced mycelial growth of the fungal pathogens. Tithonia diversifolia extract inhibited mycelial growth of C. arachidicola by 96.17% while C. odorata extract reduced mycelial growth of A. alternata by 90.74%. Tridax procumbens extract suppressed mycelial growth of C. personatum by 92.4% at 7 days incubation. Chromolaena odorata extract reduced sporulation density of C. arachidicola by 81.16% while extract of T. diversifolia induced 81.8% reduction on sporulation density of A. alternata. T. diversifolia extract also curtailed sporulation density of C. personatum by 78.32%. Fungitoxicity attributable to ultraviolet light-sterilization of extracts was comparable to that of autoclave sterilization in all the pathogens. The study revealed that plant extracts can effectively control Cercospora leaf spot disease of groundnut and its causative organisms. However, T. diversifolia C. odorata and T. procumbens, should be used as a potential biocide in plant disease management, as they showed fungicidal and fungitoxic ability.
