Determination of quinolone antibiotics by biomass waste-monolithic spin columns-solid phase extraction coupled with liquid chromatography
A simple, green and rapid method for the simultaneous determination of three kinds of quinolone antibiotics (QNs) from environmental water samples and biological samples was established by monolithic spin columns-solid phase extraction coupled with high performance liquid chromatography (MSC-SPE-HPLC). The QNs was completely adsorbed by 300 mg spent coffee grounds as the sorbents of MSC-SPE at 1000 rpm, and eluted by 2500 μL acetonitrile and 4% acetic acid (60:40, V:V). The efficient and rapid elution was obtained under the speed of 500 rpm.