A modified CTAB DNA extraction procedure for plants belonging to the family Sapindaceae

  • strict warning: Non-static method view::load() should not be called statically in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/views.module on line 906.
  • strict warning: Declaration of views_handler_argument::init() should be compatible with views_handler::init(&$view, $options) in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/handlers/views_handler_argument.inc on line 744.
  • strict warning: Declaration of views_handler_filter::options_validate() should be compatible with views_handler::options_validate($form, &$form_state) in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/handlers/views_handler_filter.inc on line 607.
  • strict warning: Declaration of views_handler_filter::options_submit() should be compatible with views_handler::options_submit($form, &$form_state) in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/handlers/views_handler_filter.inc on line 607.
  • strict warning: Declaration of views_handler_filter_boolean_operator::value_validate() should be compatible with views_handler_filter::value_validate($form, &$form_state) in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/handlers/views_handler_filter_boolean_operator.inc on line 159.
  • strict warning: Non-static method view::load() should not be called statically in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/views.module on line 906.
  • strict warning: Non-static method view::load() should not be called statically in /home4/vibu/public_html/journalijdr.com/sites/all/modules/views/views.module on line 906.
Author: 
Amzad Basha Kolar, Abdul Khayum and Sabiha Sultana, M.
Abstract: 

A good DNA extraction procedure for the isolation of genomic DNA should yield adequate and intact DNA of reasonable purity. The procedure should also be quick, simple, and cheap and if possible should avoid the use of dangerous chemicals. This paper describes a simple, rapid and efficient method for isolating genomic DNA extraction methods for mature leaves, resting buds and seedling leaves of 5 different genera namely Sapindus, Cardiospermum, Litchi, Allophilus, Dodonea of Sapindaceae. The plants belonging to this family is considered to be a “difficult” for DNA isolation due to its high polyphenolic content, which may interfere with the DNA purity especially for subsequent manipulations. This modified CTAB protocol include the use of 2M NaCl, 2% Polyvinyl pyrrolidone (PVP), 5% mercaptoethanol and 80% ethanol in the extraction as well as reducing the centrifugation times during the separation and precipitation of the DNA. Isolated genomic DNA showed high purity and high quantity. The DNA was digested by resctriction endonuleases and it was suitable for subsequent PCR amplification.

Download PDF: 

CHIEF EDITOR

  

           Prof. Dr. Bilal BİLGİN

Call for Papers - 2017

    submit your paper now

   Vol. 07, Issue 03, March 2017

CURRENT ISSUE

 

Article Tracking

IMPACT FACTOR 2016

          4.753

Get your Certificate

Copyright © 2016 International Journal Development Research. All Rights Reserved.