Monitoring the oxidative stress in hairdressers' in exposure to the vapors from the dyes of the hair, by analysis of salivary malondialdialdeyde with the use of Dipsticks

OBJECTIVES: The objective of this study is determinate with a semi quantitative method (dipsticks), the oxidative stress, produced by exposure to dyes of hairdressers, measuring the concentration of salivary malondialdehyde (MDA). MATERIALS AND METHODS: Saliva samples are provided by the hairdressers and a control group. The values of MDA are determinate using dipsticks, in a range ≤ 3 and ≥ 7 nM / ml, and For 14 random samples of saliva, the MDA, was also determined spectrophotometrically. The date is statistically examined by Univariate (UVA) and Multivariate (MVA) analysis method RESULTS: The values of MDA determined with dipsticks are statistically correlated with those obtained spectrophoto metrically, p≤0. 05. For the control group, at UVA) no variable is significant, (p≥0.05), and at MVA, age and smoking significantly increase the levels of MDA (p≤0.05). For hairdressers group at UVA, the age and the place of work increase the MDA. (p≤0.05) and at MVA only the place of work remains significant. (≤0.05). At MVA, besides the type of work, also the age and smoking significantly increase the level of MDA. DISCUSSION: The data shows in hairdressers a significant increase in salivary MDA as a function of age, and smoke, then control sample the main increase is for hairdressers regardless of their occupational work. For the direct exposure to vapors arising from hair dyes by a proportional intake of free radicals and MDA, that are also generated with increasing age. and smoking CONCLUSIONS: The saliva test with dipsticks is reliable to determine the degree of oxidative stress, especially for the oral cavity, in the professional exposure of hairdressers