Monitoring the oxidative stress in hairdressers' in exposure to the vapors from the dyes of the hair, by analysis of salivary malondialdialdeyde with the use of Dipsticks

Menicagli, R., Duca, M., Rancoita, P.M. and Arizzi, C.

OBJECTIVES: The objective of this study is determinate with a semi quantitative method (dipsticks), the oxidative stress, produced by exposure to dyes of hairdressers, measuring the concentration of salivary malondialdehyde (MDA). MATERIALS AND METHODS: Saliva samples are provided by the hairdressers and a control group. The values of MDA are determinate using dipsticks, in a range ≤ 3 and ≥ 7 nM / ml, and For 14 random samples of saliva, the MDA, was also determined spectrophotometrically. The date is statistically examined by Univariate (UVA) and Multivariate (MVA) analysis method RESULTS: The values of MDA determined with dipsticks are statistically correlated with those obtained spectrophoto metrically, p≤0. 05. For the control group, at UVA) no variable is significant, (p≥0.05), and at MVA, age and smoking significantly increase the levels of MDA (p≤0.05). For hairdressers group at UVA, the age and the place of work increase the MDA. (p≤0.05) and at MVA only the place of work remains significant. (≤0.05). At MVA, besides the type of work, also the age and smoking significantly increase the level of MDA. DISCUSSION: The data shows in hairdressers a significant increase in salivary MDA as a function of age, and smoke, then control sample the main increase is for hairdressers regardless of their occupational work. For the direct exposure to vapors arising from hair dyes by a proportional intake of free radicals and MDA, that are also generated with increasing age. and smoking CONCLUSIONS: The saliva test with dipsticks is reliable to determine the degree of oxidative stress, especially for the oral cavity, in the professional exposure of hairdressers

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           Prof. Dr. Bilal BİLGİN

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   Vol. 07, Issue 02, February 2017



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