Monitoring of oxidative stress induced by cigarette smoke by analysis salivary malondialdialdeyde with the use of dipsticks

Menicagli, R., Duca, M. and Rancoita, P. M.

Objectives: Determinate with a semi quantitative method, the oxidative stress produced by tobacco smoke, in function of the number of cigarettes and the age of the smoking, measuring the concentration of salivary malondialdehyde (MDA), using the dipsticks,
Materials and Methods: Saliva samples are provided by a smoking group, divided into three subgroups for consumption of cigarettes / day (≤ 7; 7 ÷ 20; ≥ 20), and by the control group The values of MDA are determinate using dipsticks, in a range ≤ 3 and ≥ 7 nM / ml, and in two subgroups: (≤45 and / or> 45 years old), for 14 random samples of saliva, the MDA, was also determined spectrophotometrically. The data were analyzed statistically with "Logistic Regression Method"
Results: The values of MDA determined with dipsticks are statistically correlated with those obtained spectrophotometrically, p≤0.05. For consumption of cigarettes (≥ 7 / day), the MDA concentration have an increase statistically significant compared to the control: p ≤ 0.05, and proportional to the number of them. Cigarettes consumption ≤'7 / day not involve any increase in MDA indicative vs. control: p = 0.075.The age ≥ 45 years, in smokers as in non-smokers by cigarette consumption, significantly increase the MDA, p≤0.029
Discussion: Improvement of the cigarettes results in a proportional intake of free radicals and MDA, resulting in lipid peroxidation, which is also generated with increasing age.
Conclusions: The saliva test with dipsticks is reliable to determine the degree of oxidative stress, especially for the oral cavity, in cigarettes smoking

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