Isolation and characterization of fatty acid from peel of the pear fruit (pyrus pyrifolia) and evaluate its antioxidant activity

A fatty acid was isolated and purified from pear fruit (Pyrus pyrifolia) peel extract by using column chromatography technique and evaluated its antioxidant activity by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay and reducing power assay. The isolated compound was identified as Gadoleic acid on the basis of nuclear magnetic resonance spectroscopy and it showed the high percentage of antioxidant activity but less than the ascorbic acid. As the concentration of the compound increased the percentage of activity also increased in both the DPPH assay and reducing power assay. Thus it showed that pear is the fruit with full of phytopchemicals and having strong antioxidant activity.