Phenol degradation by immobilized cells of Pseudomonas aeruginosa MTCC 4996

A newly isolated Pseudomonas aeruginosa MTCC 4996 capable of degrading phenol Pseudomonas aeruginosa MTCC 4996 cells were immobilized with six different matrices. All the immobilized matrices showed faster degradation than free cells. Among the matrices, PUF immobilized cells showed maximum degradation followed by alginate and k-carrageenan, agarose, polyacrylamide and hen egg white. P. aeuriginosa MTCC 4996 cells immobilized with PUF were showed 100% degradation up to 1400 mg/l within 750 min. PUF immobilized non-regenerated and regenerated cells showed phenol degradation up to 150 days at constant flow rate in packed bed reactor. Regenerated cells showed 100% efficiency up to 120th days. Whereas, non-regenerated cells showed 100% efficiency of phenol degradation only up to 75 days. Then onwards, the efficiency was decreased considerably.