Optimization of a non-tissue method of agrobacterium mediated gene transfer in cowpea (vigna ungiculata l. walp.)

Agrobacterium infiltration is a common method used for effective gene transfer in many cultivated plants. In this study, the various concentrations (0.5, 1.0 and 1.5 × 109 cells ml-1) of A.tumefaciens was injected into the young leaves and germinated seeds of Cowpea by syringe method and vacuum infiltration. Then the morphological and biochemical variations were observed. The results show that the vacuum infiltration method was more efficient then the syringe infiltration. Also the survival rate of transgenic plant of non-tissue culture method was increased then the tissue culture method.