Phytochemical and invitro- antimicrobial analysis of moringa oleifera lam. leaf extract

International Journal of Development Research

Volume: 
7
Article ID: 
9766
7 pages
Research Article

Phytochemical and invitro- antimicrobial analysis of moringa oleifera lam. leaf extract

Samidha M. Pawaskar and K. C. Sasangan

Abstract: 

Objective: The present study was undertaken to evaluate the preliminary phytochemical constitution and in vitro-antimicrobial activity of Moringa oleifera Lam. leaf extract. Methods: Preliminary phytochemical screening of the freshly prepared plant leaf extract was studied. The in vitro-antimicrobial activity of the successive leaf extracts of Moringa oleifera Lam. was studied in petroleum ether, chloroform, ethyl acetate, acetone, ethanol, methanol and water, against various gram positive & gram negative bacterial strains using zone of inhibition. Both Agar well diffusion method & Agar disc diffusion method were used to evaluate the antibacterial efficacy. The Minimum inhibitory concentration (MIC) of all these solvent extracts of said plant was determined by Agar well diffusion method. The reference antibiotics Chloramphenicol & Ampicillin (Antibacterial); Nystatin & Clotrimazole (Antifungal) were also tested against these standard microorganisms used in the assay and the results were compared with that of the plant extracts. Results: Preliminary phytochemical screening of the freshly prepared plant leaf extract showed the presence of alkaloids, tannins, saponins, flavonoids, glycosides, phenolic compounds, terpenoids and steroids. The in vitro-antimicrobial activity study showed that all the seven successive extracts of the leaf powder of Moringa oleifera Lam., exhibited prominent antimicrobial and antifungal activity against all microorganisms used in the study. Highly polar solvents ie. ethanol, methanol and water showed the most significant antibacterial and antifungal activity against all tested organisms. Conclusion: The results of the study revealed that the leaf powder of Moringa oleifera Lam. can be considered as a possible source of various phytochemical constituents having an in-vitro antimicrobial potential.

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