Caries dentalis one of the most common human disease. One of the bacteria considered thata very important role in the mechanism of plaque formation and increased colonization of bacteria that cause caries is Streptococcus mutans. S. mutans is considered as bacteria that causes caries dentaldue to their ability to form biofilm on the tooth surface. Searching of bioactive compounds from natural materials is still the alternative, one of the plants that has potential is Myrmecodia pendans. In previous studies, the ethyl acetate extract of M. pendans reported can inhibit the growth of S. mutans. This study aims to isolate terpenoid compound from ethyl acetate fraction ofM. pendans which has potential against S. mutans. Separation and purification of compounds using a combination of chromatographic techniques.Characterization of compounds using UV, IR, NMR and MS. Antibacterial activity test using Kirby Bauer and micro dillusion method. The results showed that the isolated compound was terpenoids type labdane diterpene. Terpenoid sensitivity test results of the concentration 10000; 5000 and 2000 μg/mL were 17.9; 16.8; 13.6 mm respectively. MIC test results and MBC are 78.125 and 625 μg/mL respectively.
Prof. Dr. Bilal BİLGİN