Premature rupture of membrane and molecular and microbiological profile of blood of newborns with suspected neonatal infection

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International Journal of Development Research

Volume: 
7
Article ID: 
10659
5 pages
Research Article

Premature rupture of membrane and molecular and microbiological profile of blood of newborns with suspected neonatal infection

Kelly L. A. Appel, Almir de S. Martins, Anna M. D. Miglioli, Paula C. N. Xavier, Albert S. de Souza, Walter P. da Silva Júnior, Rodrigo J. Oliveira, Valter A. do Nascimento and Durval B. Palhares

Abstract: 

Many term and preterm newborns with clinical signs of sepsis, whose mothers had premature rupture of membranes (PROM), have negative blood cultures, but in molecular biology research the presence of genomic DNA from different congenital bacteria has been observed. Thus, the association of molecular examination with rtPCR with different bacterial primers may help us in a more objective treatment. The samples 101 newborns with PROM were analyzed by rtPCR with primers for bacteria Streptococcus agalactie, Escherichia coli and Klebsiella pneumoniae. Blood culture results were obtained from newborn records. The mean PROM time in preterm and term pregnancies was 72.87 ± 101.85 hours and 48.70 ± 84.14 hours, respectively. The blood culture results were negative for the analyzed bacteria. No statistical association was seen of PROM time with the presence of bacterial genomic DNA (93.1%), but the clinical manifestations do suggest association with neonatal sepsis. Escherichia coli prevailed (64.3%), followed by Streptococcus agalactiae (19.8%) and Klebsiella pneumoniae (2.0%) by means of the Polimerase Chain Reaction (qPCR). Clinical and physiological findings suggest that PROM is associated with the clinical manifestations of sepsis and the presence of genomic DNA from pathogenic bacteria responsible for the occurrence of neonatal infection in preterm and term newborns.

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