Preventive use of resveratrol increases its antioxidant capacity in neuro-2a cells

Cerebral aging is a phenomenon determined almost exclusively by oxidative stress, accumulation of macromolecules damaged by oxidation and changes in the structure and function of neurons, being a risk factor for several diseases. Many data support the important role of oxidative stress in the aging process and neurodegenerative diseases, suggesting the beneficial effect of antioxidants as an adjuvant therapy. Thus, the objective of our work was to verify whether the neuroprotective effect of resveratrol is altered if N2-A cells are inserted earlier or later in an "oxidative stress environment" caused by exposure to H2O2 thus generating support for health professionals in the clinical approach. For that, we performed the production of nitric oxide and peroxynitrite, cellular reducing capacity by MTT, the production of Reactive Oxygen Species (ROS) by chemiluminescence assay and the activity of catalase and superoxide dismutase. We observed that resveratrol (5μM) in N2-A cells exerts antioxidant and neuroprotective power. This occurs mostly in the form of prevention, when the cells were pre-stimulated with resveratrol before inducing an oxidative stress environment.