Spectrophtometeric determination of lincomycin in pharmaceutical medication
International Journal of Development Research
Spectrophtometeric determination of lincomycin in pharmaceutical medication
Received 16th December, 2016; Received in revised form 14th January, 2017; Accepted 21st February, 2017; Published online 31st March, 2017
Copyright©2017, Safaa Sabri Najim. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Simple, accurate, reproducible, and inexpensive UV-Spectrophotometric methods were developed and validated for the estimation of lincomycin hydrochloride in pharmaceutical preparations. Licomycin hydrochloride was estimated at 196 nm in water. Beer’s law was obeyed in the range of (6–30), (0.-15) g/ml, (r = 0.999), (r = 0.999) in the direct and standard addition methods respectively. These methods were tested and validated for various parameters according to ICH guidelines. The detection and quantification limits were found to be (0.0506), (0.0366) μg /ml in the direct and standard addition methods respectively. The accuracy and precision of the methods were satisfactory. The proposed methods were successfully applied for the determination of lincomycin hydrochloride in pharmaceutical preparations. The results demonstrated that the procedure is precise, accurate, and recovery percentage were (R.S.D. < 1 %).
