Study of protective effect of dawa-ul-qust (a unani compound formulation) against CCL4 induced acute hepatic injury in rats

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International Journal of Development Research

Study of protective effect of dawa-ul-qust (a unani compound formulation) against CCL4 induced acute hepatic injury in rats

Abstract: 

A Unani compound formulation known as Dawa-ul-Qustwas evaluated for its hepatoprotective effects by crude as well as 50% hydro-alcoholic extract against Carbon tetra chloride (CCl4) induced liver injury in rats. The animals were divided into five groups of 6 animals each – I (Plain control), II Negative control (CCl4 treated group), III (Silymarin treated), IV DQ (Crude treated)and V DQ (Extract treated). Hepatotoxicity was induced by single administration of CCl4 (2ml/ kg I.P. 1:1 in olive oil) 24 hours before the sacrifice.The standard group was treated with Silymarin orally in the dose of 100 mg/kg body wt, once daily for 7 days.The test drug (Dawa-ul- Qust) was administered at the doses of 500 mg/kg and 74.9 mg/kg of body weight respectively in crude as well as in extract formsonce daily, per oral for 7days. On the 8thday all the animals were sacrificed by ether anaesthesia and the blood was collected for biochemical estimations and liver was dissected out for histological studies.The elevation of enzyme markers and structural changes in histological reports of liver sections were taken as the indicators of hepatic injury. The serum of each animal of all groups were estimated for, ALT, AST, and TBARS. While the liver was dissected out for histological studies to support the above parameters. The serum of each animal of all groups estimated for, the mean serum ALT, AST, and TBARS were decreased significantly as compared to CCl4 treated group. The histopathological study showed signs of recovery and regeneration in damaged liver cells as compared to CCl4 group. The study demonstrated significant hepatoprotective activity of Dawa-ul-Qust probably due to combined action of all ingredients.

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