An efficient callus induction and regeneration of Vitex negundo l. – An important medicinal plant
An efficient protocol was described for rapid callus induction and regeneration from internodal explants of Vitex negundo (Verbenaceae) which is a woody, aromatic and medicinal plant. For callus induction and regeneration, Particular plant growth regulators were used, which are auxins such as 2,4-D and NAA in combination with cytokinin BAP. The explants were cultured on Murashige and Skoog (MS, 1962) medium augmented with different concentrations of auxins and cytokinins individually and in mixture of combinations for callus induction.