Life Sciences

Antibiotic susceptibility pattern of urinary tract infection causing pathogens isolated from diabetic patients

Urinary tract infection is one of the most commonly occurring infections among the patients with diabetes mellitus. The present study was focused on the antibiotic susceptibility of the UTI pathogens isolated from the diabetic patients. About 936 UTI organisms were isolated from 900 culture positive urine samples collected from the diabetic patients attending the government hospital. The incidence of UTI was recorded to 82% among the diabetic patients for the study period of two years from March, 2011 to February, 2013. Escherichia coli was found to be the major cause of UTI.

γ-Aminobutyrate (GABA) shunt under salt stress in Saccharum officinarum

The GAD extracted from salt stress sensitive sugarcane variety CoC 671 showed increased activity with increased concentrations of NaCl treatment.  The natural GAD activity was found 141% more in salt tolerant variety Co 86032, compared to sensitive variety.  The GOT activities displayed specific induction at higher salt concentrations in CoC 671 variety.   The GOT activity was 840% more in tolerant variety than in sensitive variety and the same activity was observed at initial concentrations of salt treatment.  The activity staining of GOT and GPT did not show any difference in the banding

Gaba: Pyruvate-dependent transaminase dominates gaba: 2-oxoglutarate dependent transaminase in sugarcane and their molecular characterization

GABA occupies a significant component of cytosolic free amino acid pool.  GABA-Transaminase (GABA-T, EC 2.6.1.19), the second enzyme of GABA shunt, converts GABA to Succinic semialdehyde (SSA). GABA pyruvate- (GPT) and 2-oxoglutarate- (GOT) dependent gamma-aminobutyrate transaminase (GABA-T) activities were noted in crude leaf extract of sugarcane var. Co 86032.  The enzyme was partially purified by DEAE-Cellulose and Sephadex G-100 columns. Non-denaturing PAGE analysis of ~90 folds purified GABA-T showed 65, 60 and 55 kD proteins, which were further confirmed by activity staining.

Phytochemical evaluation of leaf extracts of Aegle marmelos

The use of traditional medicines holds a great promise as an easily available source as effective medicinal agents to cure a wide range of ailments among the people particularly in tropical developing countries like India. The present study investigates the phytochemical analysis of the major bioactive constituents of A. marmelos leaf extracts. The extractive values of aqueous, acetone and chloroform extracts were found to be 7.5 %, 5.3 % and 6.4 % respectively.

Protective role of Denolex elata against the cadmium toxicity in the glutathione peroxidase (GPX) in the fresh water fish Labeo rohita (HAMILTON)

The effect of sublethal exposure of cadmium (51.82 mg/kg) for 96 hours on various antioxidant enzymes was carried out in the freshwater fish Labeo rohita. The activity of antioxidant enzymes, such as glutathione peroxidase (GPx) were decreased was increased. This observation clearly indicates the defensive nature and the adaptive mechanism of cells against free radical induced toxicity, Denolex elata in plant extracts may afford protection from pesticide toxicity.

Identification and specification of stress proteins in the liver tissue of Carassius auratus exposed to chlorpyrifos

In recent times the enormous usage of pesticides has led to a huge deterioration of soil and consequently the aquatic ecosystem and its inhabitants. Fishes are commonly the target animals due to pesticide contamination in water bodies at large. It is being understood that the fishes create a stress response towards the entry of toxic chemicals by means of synthesizing a group of novel proteins called stress proteins which play a major role in the stress- resistance.

Curative role of caffeine on mercury induced alterations of protein levels in the, Lamellidens corrianus (LEA)

The present investigation was carried out to study the probable role of caffeine (1, 3, 7-Trimethylxanthine) on mercury induced alterations on the experimental freshwater bivalve, Lamellidens corrianus (Lea). The effect on bivalve was studied under five groups. A group bivalve were kept as control, B group bivalves were exposed to acute dose (LC50/2) of mercury (0.6 ppm equivalent to 0.444 ppm Hg++), C group bivalves were exposed to acute dose (LC50/2) of mercuric chloride with caffeine (5 mg/L.). After 4 days bivalves from group B were divided into two groups D and E.

A study of higher secondary students’ participation in environmental activities in relation to environmental awareness

The purpose of this research was to study the higher secondary students’ participation in environmental activities in relation to environmental awareness. This study correlates certain demographic variables in respect of environmental awareness and participation in environmental activities. Environmental Awareness Scale constructed and validated  by  Dr. S.

Soft landscape elements: Importance and implications on thermal control in tropical buildings

Thermal control in buildings is highly essential in the tropics where high solar radiation is received throughout the year. Its importance cannot be overemphasized in the face of climate change which is rapidly changing our world and exposing it to higher risk of environmental disasters. Climate change has been attributed to increased energy use from various human activities which cause a release of carbon dioxide and other green house gases into the atmosphere. Thermal control in buildings is one of the gate ways to minimizing the energy used for cooling.

Effects of packaging materials and methods on physical properties and food safety of duck sausage

Utilization of spent duck through sausage preparation and estimating the optimum storage condition throughout the retail chain was the objective of the present study. PET/Poly and laminate of metalized PET/Poly with polyethylene pouches under aerobic and vacuum packaging stored in refrigerator (4±1° C) and freezer (-18±1° C) condition were considered in the experiment. TBA value, pH, Tyrosine value, TPC, TPSC and YMC of the samples increased with the storage period whereas a decreasing trend in case of moisture and all the sensory parameters throughout the storage period was observed.