Time-mortality relationship in marine ornamental harlequin shrimp hymenocera picta against white spot syndrome virus using bacterially expressed recombinant envelope proteins (vp39 and vp28)
International Journal of Development Research
Time-mortality relationship in marine ornamental harlequin shrimp hymenocera picta against white spot syndrome virus using bacterially expressed recombinant envelope proteins (vp39 and vp28)
Received 24th August 2017; Received in revised form 16th September, 2017; Accepted 15th October, 2017; Published online 12th November, 2017
Copyright ©2017, Priya Lakshmi and Dr. Dhansekaran. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
WSSV is one of the most serious viral diseases affecting cultured shrimp worldwide This lethal virus causes formation of white spots in exoskeleton under carapace and can infect not only penaeid shrimps, but also other marine and freshwater crustaceans. The WSSV genome contains at five known major structural proteins: VP39, VP28, VP19, VP26, VP24 and VP15. Studies on WSS viral proteins have demonstrated that VP28 and VP19 are associated with the virion envelope. In the present study ,we investigated the efficacy of recombinant proteins VP39 and VP28 expressed in E. coli and delivered through oral administration, against WSSV infection in marine ornamental Harlequin shrimp Hymenocera picta. The DNA was extracted and amplified by PCR. Then it was purified through AGE. Then the viral innoculum was prepared for further study. The products of vp28 and vp39 genes were cloned into pET-32a (+) expression vector and transformed into E. coli BL21 (DE3) (pSBET A). To determine the expression of recombinant proteins. each shrimps of these groups was intramuscularly (IM) injected with 50μl purified recombinant proteins rVP28, rVP39, mixture of both (rVP28+rVP39) and two groups were injected with phosphate buffered saline (PBS) in the 4th or 5th tail segment of the shrimp with a 29 gauge needle as positive and negative control. The dose of recombinant proteins injected was 10 μg/g of Harlequin shrimp Hymenocera picta. After 7th day of first dose, shrimps were immunized with booster dose of same concentration of the recombinant proteins. We observed that This study demonstrates that vaccination by recombinant WSSV proteins VP28 and VP39 increased survival rate compared to the control groups in Hymenocera picta. Oral vaccinations of shrimp with rVP39 or rVP28 resulted in significantly lower mortalities than those occurred among the control animals.
