Use of polymerase chain reaction for detection of trypanosoma vivax infection in cattle

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Author: 
Neurisvan Ramos Guerra, Maria Fernanda Melo Monteiro, Hevila Mara Moreira Sandes Guerra, 1Bruno Henrique Leal e Silva Alves, *1Edson Moura da Silva, Mateus Matiuzzi da Costa and Leucio Câmara Alves
Abstract: 

Trypanosoma vivax is a protozoan that infects a wide range of wild and domestic ungulates causing important economic losses on the livestock industry. Considering a recent outbreak in bovines from the state of Pernambuco, the aim of this study was to utilize the PCR as a tool to detect T. vivax DNA in the blood of animals from different regions (i.e., Litoral, Zona da Mata, Agreste and Sertão). Based on previous serological survey, the cities in each region which had the highest frequency of reagent cattle to antibodies IgG anti-T. vivax by Immunofluorescence Antibody Test, were selected. A total of 127 bovine blood samples were obtained in heparin vacuum tube for further DNA extraction and PCR. The PCR was carried out using primers 18STnF2 and 18STnR3, which delimit a fragment of 659 bp of the 18S rRNA gene in Brazilian isolates of T. vivax. Out of 127 analyzed samples, 44.88% (57/127) presented amplicons with 659 bp compatible with T. vivax.The PCR proved to be a good tool for the diagnosis of infection by T. vivax in bovines, being important in the detection of infected animals independently from the clinical status.

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