Validation of an analysis method of 13 organochlorine pesticide residues in bivalves "oyters" using quechers extraction and gaz chromatography-tandem mass spectrometry ``gc-ms/ms``
International Journal of Development Research
Validation of an analysis method of 13 organochlorine pesticide residues in bivalves "oyters" using quechers extraction and gaz chromatography-tandem mass spectrometry ``gc-ms/ms``
Received 29th February, 2017; Received in revised form 07th March, 2017; Accepted 14th April, 2017; Published online 18th May, 2017
Copyright©2017, Fatima HABTI et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Pesticides are now recognized for their adverse effects on human health and the environment. The maximum residue limits (MRLs) for pesticides authorized in the food chain are increasingly low. Thus high-performance analytical techniques are imposed for their identification and quantification. The objective of this study is to develop a multi-residual analysis method for organochlorine pesticides in bivalves by gas chromatography coupled with triple quadrupole mass spectrometry. A global protocol comprising a QuECHERS version of the extraction step followed by a step of purification of the extract obtained on a mixture of the adsorbents was carried out. The sulfotep applied as an internal standard is excellent for controlling the various stages of sample preparation and extraction. The developed method allows the detection of 13 organochlorine pesticides in bivalves "Oyster". It is verified limeair (R≥ 0.99), specific, selective, having an average yield greater than 70% and the relative standard deviations (RSD ≤20%), repeatable, reproducible, exact and accurate. Thereby, the potential of the adopted method has been demonstrated.
