In-vitro studies on effect of methanol and water extracts of detarium microcarpum guill and perr stem bark on root-knot nematode (Meloigogyne Javanica)

Jada, M. Y., Oaya, C. S. and Reuben, K. D.

Methanol and water soluble extract of Detarium microcarpum Guill and Perr effect of Roo-knot nematode (Meloidogyne javanica) were studied in Laboratory of the Department of Crop Protection, Modibbo Adama University of Technology, Yola in 2014. Root-knot nematode (M. javanica) juvenile mortality in the Laboratory and chemical compound responsible were ascertained. The two extracts (Methanol and water) were run separately, each consisting of six treatments (100%, 80%, 60%, 40% and 20% extract concentration and distilled water as 0%) replicated four times arranged in a Completely Randomised Design placed on Laboratory benches. The dead juveniles of M. javanica were counted after 24hrs, 48hrs and 72hrs. Chemical compound extraction and identification were earlier carried out. The results indicated that root-knot nematode (M. javanica) juvenile mortality was significant at 100% concentration for both methanol and water extracts. At 100% concentration extract M. javanica juvenile mortality was 57.00 while that of water extract gave 61.1 M. javanica juvenile mortality. The chemical analysis result indicates that methanol extract contains medium concentration of Tannins (lead acetate) and very high concentration of Terpenoids. Both Tannins and Terpenoids were found in low concentration of the bark.

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