Effects Of High-Fat Diet On Adipose Tissue Andpancreatic Islet Function In Ovariectomized Rats

The increase in life expectancy allows women to live longer, with the deleterious effects of gonadal deficiency, such as the accumulation of abdominal fat; and the new habits of modern society are dedicated to eating foods rich in fats and carbohydrates, which contribute to overweight and obesity.The objective of this study was to evaluate intra-abdominal fat tissue and pancreas in ovariectomized rats. Wistar rats, 90 days-old, after evaluation by vaginal cytology were ovariectomized. The animals were divided into four groups and were fed diets with soybean oil concentrations of 4% or 19% for 30 days.Body weight and food intake was evaluated. At 120 days-old, total fat and lean mass were assessed by densitometry (DEXA) and after the blood was collected. Serum was used for determination of triglyceride, cholesterol, HDL, glucose, estradiol and insulin.Adipose tissue was excised, weighed and used to evaluate the morphology and expressionERα. Pancreas used to evaluate the morphology and immunohistochemistry by insulin.The mass of the uterus, serum estradiol were lower in OVX and OVXHf groups.The mass of intra-abdominal adipose tissue and the total body fat were higher in OVXHf group. Serum insulin triglycerides, cholesterol and glucoseincreased in ShamHf and OVXHf groups. There was an increase on area of adipocytes and islet pancreasOVX, OVXHf groups. ERα expression was lower in OVX and OVXHf.We concluded, the deficiency of estrogen associated the intake of high fat diet can be accelerating the onset of metabolic syndrome.he works in the area metabolic diseases.