Rapid in viro multiplication of some sugarcane cultivars (saccharum officnarum) via embryogenic callus culture of young leaf tissues
In this study, we have reported an efficient protocol for induction of callus and regeneration without sterliztion step of some sugarcane cultivars. Callus induction from young leaf tissues was attained from MS culture medium supplemented with different concentrations of the growth regulator substances. The results showed that the highest callus rate on MS medium supplemented with 3-4mg/l 2,4D. The highest multiplication of arpical meristem was observed on MS medium which was supplemented with BAP 2-2.5 mg/l, kinetin 0,2 mg/l, 10mg/l PVP and 15% coconut juice.