Stability indicating rp-hplc determination of curcumin in vicco turmeric cream and kasturi turmeric churna
A simple, specific, precise and stability-indicating RP-HPLC method was developed and validated for analysis of Curcumin in bulk drug, cream and churna formulations. Chromatographic separation were achieved using Agilent-TC C18 column (250 X 4.6 mm; 5µ) column at ambient temperature. Mixture of methanol, acetonitrile, and 5% acetic acid (35: 50: 15, v/v) was used as mobile phase at constant flow rate of 1.0 ml/min and 420 nm was selected as wave length for detection of method. The Curcumin peak was obtained at RT 4.92 min.