In vitro anti-cervical cancer effect of proteoglycans isolated from deep sea shark cartilage by inducing apoptosis and cell cycle arrest
The present study was aimed at studying in vitro anti-cervical cancer effect of proteoglycans (PGs) isolated from deep sea shark. Anti-proliferative activity and apoptotic involvement were confirmed by following assays viz, the cell viability assay by MTT assay, morphological analysis and colony formation assay. The sample showed a significant cytotoxic activity for HeLa cells and results showed that the PGs at a concentration of 250µg/mL inhibit the colony formation capacity. Apoptosis induction was confirmed by the chromatin condensation assay by Hoechst 33342 nuclear staining.